General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 GPR182 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0754C2)
The PathHunter GPR182 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR182 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR182 and EA-tagged β-Arrestin-2. Activation of GPR182 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS CALCR (No RAMP) β-Arrestin Cell Line, 2 vials. (93-0566C3)
The PathHunter CALCR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCR and EA-tagged β-Arrestin-2. Activation of CALCR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS rhTrkA-rhP75 Functional Assay, 2 vials. (93-1009C3)
The PathHunter U2OS rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rhesus version of the protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter CHO-K1 GPR18 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0560C2A)
The PathHunter GPR18 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR18 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR18 and EA-tagged β-Arrestin-2. Activation of GPR18 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GPR55 β-Arrestin Cell Line, 2 vials. (93-0245C2)
The PathHunter GPR55 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. lysophosphatidylinositol) based activation of GPR55 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR55 and EA-tagged β-Arrestin-2. Activation of GPR55 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC MCF7-Luc2; Breast Adenocarcinoma; Human (Homo sapiens)
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
ATCC hTERT immortalIzed; dermal melanocytes; Human(Homo sapiens)
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
Eurofins Discoverx PathHunter CHO-K1 RARα Protein Interaction Cell Line, 2 vials. (93-0325C2)
The PathHunter RARα Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. retinoic acid) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. The included cell line overexpresses ProLink-tagged RARα NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of RARα, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
Eurofins Discoverx cAMP Hunter CHO-K1 DRD2S (Short Isoform) Gi Cell Line, 2 vials. (95-0084C2)
The cAMP Hunter DRD2S Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. dopamine) based activation of DRD2S GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD2S, a naturally Gi/Go coupled GPCR. Upon activation of DRD2S, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 rCRTH2 β-Arrestin Cell Line, 2 vials. (93-0580C2)
The PathHunter rCRTH2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin D2) based activation of rCRTH2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCRTH2 and EA-tagged β-Arrestin-2. Activation of rCRTH2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter HEK 293 SSTR3 β-Arrestin Cell Line, 2 vials. (93-0244C1)
The PathHunter SSTR3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. somatostatin) based activation of SSTR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR3 and EA-tagged β-Arrestin-2. Activation of SSTR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Promocell GmbH PromoCell HPAEC Pellet from > 1 million Normal Human Pulmonary Artery Endothelial Cells
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Eurofins Discoverx PathHunter CHO-K1 MR Protein Interaction Cell Line, 2 vials. (93-0758C2)
The PathHunter MR Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. aldosterone) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. The included cell line overexpresses ProLink-tagged MR (mineralocorticoid receptor) NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of MR, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
Eurofins Discoverx cAMP Hunter CHO-K1 rDRD2S (Short Isoform) Gi Cell Line, 2 vials. (95-0129C2)
The cAMP Hunter rDRD2S Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. dopamine, pergolide) based activation of rDRD2S GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type rDRD2S, a naturally Gi/Go coupled GPCR. Upon activation of rDRD2S, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS TGFBR1/TGFBR2/ENG Dimerization Cell Line, 2 vials. (93-1122C3)
The PathHunter TGFBR1/TGFBR2/ENG Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. TGF-β1) induced dimerization of TGFBR1/TGFBR2/ENG receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside TGFBR2 target protein fused to EA and untagged ENG. Upon ligand-induced dimerization of TGFBR1/TGFBR2/ENG, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.